Source - Structure - Sequence with comments - Sequence only - References
Source
pCMV/SEAP Expression Vector, Catalog Number: AV10C
(Tropix Inc., 47 Wiggins Avenue, Bedford, Massachusetts, 01730,
Phone: 1-800-542-2369, Fax: 1-617-275-8581)
Structure
Berger et al. started with the gene
of the membrane bound human placental alkaline phosphatase
(PLAP). The secreted form (SEAP) was derived by insertion of a
translational terminator (stop codon) after aa 489 using an
oligonucleotide-directed mutagenesis procedure. This procedure
generated a unique Hpa I site. A 2.1 kb Hind III/EcoR I fragment
containing the SEAP gene was transferred from pBC/PL/SEAP (Berger et al. 1988) to pBluescript
IIKS+. A 2.1 kb Hind III / Xba I fragment was then transferred
into the pRc/CMV plasmid to form the pCMV/SEAP plasmid vector
(Total 7582 bases).
cmvseap (length: 7582)
1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTCGACTCT CAGTACAATC 51 TGCTCTGATG CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT 101 GGAGGTCGCT GAGTAGTGCG CGAGCAAAAT TTAAGCTACA ACAAGGCAAG 151 GCTTGACCGA CAATTGCATG AAGAATCTGC TTAGGGTTAG GCGTTTTGCG 201 CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT GATTATTGAC 251 TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG 351 CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT 401 AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAC TATTTACGGT 451 AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC 501 CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA 551 CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA 651 TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA 701 TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA 751 ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG 801 GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTAACT 851 GGCTTATCGA AATTAATACG ACTCACTATA GGGAGACCCA AGCTTCTGCA 901 TGCTGCTGCT GCTGCTGCTG CTGGGCCTGA GGCTACAGCT CTCCCTGGGC 951 ATCATCCCAG TTGAGGAGGA GAACCCGGAC TTCTGGAACC GCGAGGCAGC 1001 CGAGGCCCTG GGTGCCGCCA AGAAGCTGCA GCCTGCACAG ACAGCCGCCA 1051 AGAACCTCAT CATCTTCCTG GGCGATGGGA TGGGGGTGTC TACGGTGACA 1101 GCTGCCAGGA TCCTAAAAGG GCAGAAGAAG GACAAACTGG GGCCTGAGAT 1151 ACCCCTGGCC ATGGACCGCT TCCCATATGT GGCTCTGTCC AAGACATACA 1201 ATGTAGACAA ACATGTGCCA GACAGTGGAG CCACAGCCAC GGCCTACCTG 1251 TGCGGGGTCA AGGGCAACTT CCAGACCATT GGCTTGAGTG CAGCCGCCCG 1301 CTTTAACCAG TGCAACACGA CACGCGGCAA CGAGGTCATC TCCGTGATGA 1351 ATCGGGCCAA GAAAGCAGGG AAGTCAGTGG GAGTGGTAAC CACCACACGA 1401 GTGCAGCACG CCTCGCCAGC CGGCACCTAC GCCCACACGG TGAACCGCAA 1451 CTGGTACTCG GACGCCGACG TGCCTGCCTC GGCCCGCCAG GAGGGGTGCC 1501 AGGACATCGC TACGCAGCTC ATCTCCAACA TGGACATTGA CGTGATCCTA 1551 GGTGGAGGCC GAAAGTACAT GTTTCCCATG GGAACCCCAG ACCCTGAGTA 1601 CCCAGATGAC TACAGCCAAG GTGGGACCAG GCTGGACGGG AAGAATCTGG 1651 TGCAGGAATG GCTGGCGAAG CGCCAGGGTG CCCGGTATGT GTGGAACCGC 1701 ACTGAGCTCA TGCAGGCTTC CCTGGACCCG TCTGTGACCC ATCTCATGGG 1751 TCTCTTTGAG CCTGGAGACA TGAAATACGA GATCCACCGA GACTCCACAC 1801 TGGACCCCTC CCTGATGGAG ATGACAGAGG CTGCCCTGCG CCTGCTGAGC 1851 AGGAACCCCC GCGGCTTCTT CCTCTTCGTG GAGGGTGGTC GCATCGACCA 1901 TGGTCATCAT GAAAGCAGGG CTTACCGGGC ACTGACTGAG ACGATCATGT 1951 TCGACGACGC CATTGAGAGG GCGGGCCAGC TCACCAGCGA GGAGGACACG 2001 CTGAGCCTCG TCACTGCCGA CCACTCCCAC GTCTTCTCCT TCGGAGGCTA 2051 CCCCCTGCGA GGGAGCTCCA TCTTCGGGCT GGCCCCTGGC AAGGCCCGGG 2101 ACAGGAAGGC CTACACGGTC CTCCTATACG GAAACGGTCC AGGCTATGTG 2151 CTCAAGGACG GCGCCCGGCC GGATGTTACC GAGAGCGAGA GCGGGAGCCC 2201 CGAGTATCGG CAGCAGTCAG CAGTGCCCCT GGACGAAGAG ACCCACGCAG 2251 GCGAGGACGT GGCGGTGTTC GCGCGCGGCC CGCAGGCGCA CCTGGTTCAC 2301 GGCGTGCAGG AGCAGACCTT CATAGCGCAC GTCATGGCCT TCGCCGCCTG 2351 CCTGGAGCCC TACACCGCCT GCGACCTGGC GCCCCCCGCC GGCACCACCG 2401 ACGCCGCGCA CCCGGGTTAA CCCGTGGTCC CCGCGTTGCT TCCTCTGCTG 2451 GCCGGGACCC TGCTGCTGCT GGAGACGGCC ACTGCTCCCT GAGTGTCCCG 2501 TCCCTGGGGC TCCTGCTTCC CCATCCCGGA GTTCTCCTGC TCCCCACCTC 2551 CTGTCGTCCT GCCTGGCCTC CAGCCCGAGT CGTCATCCCC GGAGTCCCTA 2601 TACAGAGGTC CTGCCATGGA ACCTTCCCCT CCCCGTGCGC TCTGGGGACT 2651 GAGCCCATGA CACCAAACCT GCCCCTTGGC TGCTCTCGGA CTCCCTACCC 2701 CAACCCCAGG GACTGCAGGT TGTGCCCTGT GGCTGCCTGC ACCCCAGGAA 2751 AGGAGGGGGC TCAGGCCATC CAGCCACCAC CTACAGCCCA GTGGCCTCGA 2801 GGGATCCGCT TCCTGCCCCT GCTGGCCCTG CTCATCCTCT GGGAGCCCCG 2851 CCCTGCCCAG GCTTTTGTCA AACAGCACCT TTGTGGTTCT CACTTGGTGG 2901 AAGCTCTCTA CCTGGTGTGT GGGGAGCGTG GATTCTTCTA CACACCCATG 2951 TCCCGCCGCG AAGTGGAGGA CCCACAAGGT AAGCTCTGCT CCTGAATTCC 3001 TGCAGCCCGG GGGATCCACT AGTTCTAGAG GGCCCTATTC TATAGTGTCA 3051 CCTAAATGCT AGAGCTCGCT GATCAGCCTC GACTGTGCCT TCTAGTTGCC 3101 AGCCATCTGT TGTTTGCCCC TCCCCCGTGC CTTCCTTGAC CCTGGAAGGT 3151 GCCACTCCCA CTGTCCTTTC CTAATAAAAT GAGGAAATTG CATCGCATTG 3201 TCTGAGTAGG TGTCATTCTA TTCTGGGGGG TGGGGTGGGG CAGGACAGCA 3251 AGGGGGAGGA TTGGGAAGAC AATAGCAGGC ATGCTGGGGA TGCGGTGGGC 3301 TCTATGGCTT CTGAGGCGGA AAGAACCAGC TGGGGCTCGA GGGGGGATCC 3351 CCACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC 3401 GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 3451 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT 3501 AAATCGGGGC ATCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG 3551 ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 3601 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG 3651 TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT 3701 CTTTTGATTT ATAAGGGATT TTGGGGATTT CGGCCTATTG GTTAAAAAAT 3751 GAGCTGATTT AACAAAAATT TAACGCGAAT TTTAACAAAA TATTAACGTT 3801 TACAATTTAA ATATTTGCTT ATACAATCTT CCTGTTTTTG GGGCTTTTCT 3851 GATTATCAAC CGGGGTGGGT ACCGAGCTCG AATTCTGTGG AATGTGTGTC 3901 AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGGCAGGCA GAAGTATGCA 3951 AAGCATGCAT CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT 4001 CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC 4051 ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC 4101 CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG 4151 CCGAGGCCGC CTCGGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT 4201 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GGATATCCAT 4251 TTTCGGATCT GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC 4301 AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC 4351 GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT 4401 CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT 4451 CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG 4501 GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC 4551 GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT 4601 CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG 4651 CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC 4701 GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG 4751 ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG 4801 TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC 4851 CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT 4901 CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG CTATCAGGAC 4951 ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC 5001 TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA 5051 TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT 5101 TCGAAATGAC CGACCAAGCG ACGCCCAACC TGCCATCACG AGATTTCGAT 5151 TCCACCGCCG CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA 5201 CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG GAGTTCTTCG 5251 CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT 5301 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG 5351 TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGG ATCCCGTCGA 5401 CCTCGAGAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT 5451 GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT 5501 AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG 5551 CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT 5601 GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC 5651 GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC 5701 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG 5751 ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC 5801 CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 5851 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG 5901 GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT 5951 CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 6001 GGGAAGCGTG GCGCTTTCTC AATGCTCACG CTGTAGGTAT CTCAGTTCGG 6051 TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG 6101 CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 6151 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA 6201 GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC 6251 TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 6301 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA 6351 CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA 6401 AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 6451 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA 6501 AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA 6551 ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT 6601 CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG 6651 CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT 6701 GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA 6751 TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC 6801 CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT 6851 AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC 6901 TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT 6951 CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA 7001 AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC 7051 CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG 7101 TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG 7151 TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC 7201 AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA 7251 TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG 7301 AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC 7351 TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG 7401 CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC 7451 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG 7501 CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC 7551 GCACATTTCC CCGAAAAGTG CCACCTGACG TC //
Sequence only: SEAP
GACGGATCGGGAGATCTCCCGATCCCCTATGGTCGACTCTCAGTACAATC TGCTCTGATGCCGCATAGTTAAGCCAGTATCTGCTCCCTGCTTGTGTGTT GGAGGTCGCTGAGTAGTGCGCGAGCAAAATTTAAGCTACAACAAGGCAAG GCTTGACCGACAATTGCATGAAGAATCTGCTTAGGGTTAGGCGTTTTGCG CTGCTTCGCGATGTACGGGCCAGATATACGCGTTGACATTGATTATTGAC TAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATA TGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCG CCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGT AACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGACTATTTACGGT AAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCC CCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTA CATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCA TCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGA TAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAA TGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTA ACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAG GTCTATATAAGCAGAGCTCTCTGGCTAACTAGAGAACCCACTGCTTAACT GGCTTATCGAAATTAATACGACTCACTATAGGGAGACCCAAGCTTCTGCA TGCTGCTGCTGCTGCTGCTGCTGGGCCTGAGGCTACAGCTCTCCCTGGGC ATCATCCCAGTTGAGGAGGAGAACCCGGACTTCTGGAACCGCGAGGCAGC CGAGGCCCTGGGTGCCGCCAAGAAGCTGCAGCCTGCACAGACAGCCGCCA AGAACCTCATCATCTTCCTGGGCGATGGGATGGGGGTGTCTACGGTGACA GCTGCCAGGATCCTAAAAGGGCAGAAGAAGGACAAACTGGGGCCTGAGAT ACCCCTGGCCATGGACCGCTTCCCATATGTGGCTCTGTCCAAGACATACA ATGTAGACAAACATGTGCCAGACAGTGGAGCCACAGCCACGGCCTACCTG TGCGGGGTCAAGGGCAACTTCCAGACCATTGGCTTGAGTGCAGCCGCCCG CTTTAACCAGTGCAACACGACACGCGGCAACGAGGTCATCTCCGTGATGA ATCGGGCCAAGAAAGCAGGGAAGTCAGTGGGAGTGGTAACCACCACACGA GTGCAGCACGCCTCGCCAGCCGGCACCTACGCCCACACGGTGAACCGCAA CTGGTACTCGGACGCCGACGTGCCTGCCTCGGCCCGCCAGGAGGGGTGCC AGGACATCGCTACGCAGCTCATCTCCAACATGGACATTGACGTGATCCTA GGTGGAGGCCGAAAGTACATGTTTCCCATGGGAACCCCAGACCCTGAGTA CCCAGATGACTACAGCCAAGGTGGGACCAGGCTGGACGGGAAGAATCTGG TGCAGGAATGGCTGGCGAAGCGCCAGGGTGCCCGGTATGTGTGGAACCGC ACTGAGCTCATGCAGGCTTCCCTGGACCCGTCTGTGACCCATCTCATGGG TCTCTTTGAGCCTGGAGACATGAAATACGAGATCCACCGAGACTCCACAC TGGACCCCTCCCTGATGGAGATGACAGAGGCTGCCCTGCGCCTGCTGAGC AGGAACCCCCGCGGCTTCTTCCTCTTCGTGGAGGGTGGTCGCATCGACCA TGGTCATCATGAAAGCAGGGCTTACCGGGCACTGACTGAGACGATCATGT TCGACGACGCCATTGAGAGGGCGGGCCAGCTCACCAGCGAGGAGGACACG CTGAGCCTCGTCACTGCCGACCACTCCCACGTCTTCTCCTTCGGAGGCTA CCCCCTGCGAGGGAGCTCCATCTTCGGGCTGGCCCCTGGCAAGGCCCGGG ACAGGAAGGCCTACACGGTCCTCCTATACGGAAACGGTCCAGGCTATGTG CTCAAGGACGGCGCCCGGCCGGATGTTACCGAGAGCGAGAGCGGGAGCCC CGAGTATCGGCAGCAGTCAGCAGTGCCCCTGGACGAAGAGACCCACGCAG GCGAGGACGTGGCGGTGTTCGCGCGCGGCCCGCAGGCGCACCTGGTTCAC GGCGTGCAGGAGCAGACCTTCATAGCGCACGTCATGGCCTTCGCCGCCTG CCTGGAGCCCTACACCGCCTGCGACCTGGCGCCCCCCGCCGGCACCACCG ACGCCGCGCACCCGGGTTAACCCGTGGTCCCCGCGTTGCTTCCTCTGCTG GCCGGGACCCTGCTGCTGCTGGAGACGGCCACTGCTCCCTGAGTGTCCCG TCCCTGGGGCTCCTGCTTCCCCATCCCGGAGTTCTCCTGCTCCCCACCTC CTGTCGTCCTGCCTGGCCTCCAGCCCGAGTCGTCATCCCCGGAGTCCCTA TACAGAGGTCCTGCCATGGAACCTTCCCCTCCCCGTGCGCTCTGGGGACT GAGCCCATGACACCAAACCTGCCCCTTGGCTGCTCTCGGACTCCCTACCC CAACCCCAGGGACTGCAGGTTGTGCCCTGTGGCTGCCTGCACCCCAGGAA AGGAGGGGGCTCAGGCCATCCAGCCACCACCTACAGCCCAGTGGCCTCGA GGGATCCGCTTCCTGCCCCTGCTGGCCCTGCTCATCCTCTGGGAGCCCCG CCCTGCCCAGGCTTTTGTCAAACAGCACCTTTGTGGTTCTCACTTGGTGG AAGCTCTCTACCTGGTGTGTGGGGAGCGTGGATTCTTCTACACACCCATG TCCCGCCGCGAAGTGGAGGACCCACAAGGTAAGCTCTGCTCCTGAATTCC TGCAGCCCGGGGGATCCACTAGTTCTAGAGGGCCCTATTCTATAGTGTCA CCTAAATGCTAGAGCTCGCTGATCAGCCTCGACTGTGCCTTCTAGTTGCC AGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGT GCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTG TCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCA AGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGC TCTATGGCTTCTGAGGCGGAAAGAACCAGCTGGGGCTCGAGGGGGGATCC CCACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGC GCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCT TTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCT AAATCGGGGCATCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCG ACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCC TGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAG TGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATT CTTTTGATTTATAAGGGATTTTGGGGATTTCGGCCTATTGGTTAAAAAAT GAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTT TACAATTTAAATATTTGCTTATACAATCTTCCTGTTTTTGGGGCTTTTCT GATTATCAACCGGGGTGGGTACCGAGCTCGAATTCTGTGGAATGTGTGTC AGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGGCAGGCAGAAGTATGCA AAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCT CCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACC ATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTC CGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGG CCGAGGCCGCCTCGGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTT TTTGGAGGCCTAGGCTTTTGCAAAAAGCTCCCGGGAGCTTGGATATCCAT TTTCGGATCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAAC AAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTC GGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTT CCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGT CCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTG GCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGC GGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGT CATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATG CGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGC GAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCG ATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTG TTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGAC CCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTT CTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGAC ATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGC TGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCA TCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAGCGGGACTCTGGGGT TCGAAATGACCGACCAAGCGACGCCCAACCTGCCATCACGAGATTTCGAT TCCACCGCCGCCTTCTATGAAAGGTTGGGCTTCGGAATCGTTTTCCGGGA CGCCGGCTGGATGATCCTCCAGCGCGGGGATCTCATGCTGGAGTTCTTCG CCCACCCCAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAAT AGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTG TGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGGATCCCGTCGA CCTCGAGAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATT GTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGT AAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCG CTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAAT GAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCC GCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGC GGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGG ATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAAC CGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGA CGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAG GACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCT CCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTC GGGAAGCGTGGCGCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTCGG TGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAG CCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGT AAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCA GAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAAC TACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCC AGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCA CCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGA AAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGC TCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAA AAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCA ATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAAT CAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTG CCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCT GGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGA TTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTC CTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCT AGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGC TACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCT CCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAA AAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGC CGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTG TCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAG TCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTC AATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCA TTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTG AGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATC TTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATG CCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTC TTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAG CGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGC GCACATTTCCCCGAAAAGTGCCACCTGACGTC
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