Tris Stock Solution, 1 M

Reagent Final Concentration Volume Mass
Tris base 1 M   12.11 g
H2O   80 ml  

Dissolve Tris, pH with conc. HCl (see table below), H2O QS to 100 ml

pH HCl
7.4 7ml
7.6 6ml
8.0 4.2ml

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