| Reagent | Final Conc. | Volume | Mass |
| Tris base (FW 121.14) | 0.2 M | 12.114 g | |
| NaCl (FW 58.44) | 1.5 M | 43.83 g | |
| H2O | QS to 500 mL |
adjust pH to 7.2 - 7.4 with HCl
for TBST add Tween 20 (Polyoxyethylene sorbitane monolaureate)
to 0.05%.
For 2 L use 200 mL 10 x TBS and 1 mL Tween 20.