| Reagent | Final Conc | Volume | Mass |
| Tris base | 30.285 g | ||
| Na2EDTA-2H2O | 1.86 g | ||
| Boric Acid | 13.25 g | ||
| H2O | 800 mL |
Adjust pH to 8.3 with boric acid. Adjust volume to 1 L with H2O.
| Reagent | Final Conc | Volume | Mass |
| Tris base | 121.14 g | ||
| Na2EDTA-2H2O | 7.45 g | ||
| Boric Acid | 53.03 g | ||
| H2O | 3800 mL |
Adjust pH to 8.3 with boric acid. Adjust volume to 4 L with H2O.