TAE Buffer Stock Solution, 50 x
Reagent
Final Concentration
Volume
Mass
Tris base
40 mM
121 g
glacial acetic acid
28.55 ml
0.5 M EDTA stock, pH 8.0
1 mM
50 ml
dH
2
O
400 ml
add dH
2
O to 500 ml total volume
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