SOB-medium

Reagent Final Conc. Volume Mass
dH20   900 ml  
Bacto Tryptone     20 g
Bacto Yeast Extract     5 g
NaCl (MW 58.44) 10.0 mM   0.5844 g
KCl 2.5 mM   0.1865 g

QS to 1 liter, adjust pH to 7.0 with NaOH, autoclave

Reagent Final Conc. Volume Mass
dH20   400 ml  
Bacto Tryptone     10 g
Bacto Yeast Extract     2.5 g
NaCl (MW 58.44) 10.0 mM   0.2922 g
KCl 2.5 mM   0.09325 g

QS to 0.5 liter, adjust pH to 7.0 with NaOH, autoclave


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