SM Buffer

Reagent Final Conc. Volume Mass
H2O   800 ml  
NaCl (MW 58.44, Fisher S271-3)     5.8 g
MgSO4 6H20 (MW 203.31, Fisher M33-500)     2 g
1 M Tris-Cl pH 7.5   50 ml  
2% gelatin   5 ml  

QS to 1 liter, autoclave

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