SDS-PAGE 4 x Sample Buffer, reducing

Reagent Final Conc Volume Mass
80% Glycerol 40% 5 mL  
1 M Tris-Cl, pH6.8 240 mM 2.4 mL  
SDS 8%   0.8 g
bromophenol blue 0.1%   4 mg
H2O   2.1 mL  

Before use add 1/20 volumes of 2-mercaptoethanol.

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