RPMI 1640 (media for YAC-1 cells)

Reagent	Final Conc.	Volume	Mass
dH₂O		800 ml
RPMI 1640 powdered media (Sigma R-6504)			1 bottle
NaHCO₃			2.2 g
L-glutamine 200 mM solution (Sigma G7513)	2 mM	10 ml

adjust volume to 1 L
adjust pH to 7.2
sterile filter
add 10 ml pen/strep (100x) to a final concentration of 100 U/ml penicillin, 100 µg/ml streptomycin
add 10 % FBS