RIPA buffer (cell lysis buffer 1)

Reagent	Final Conc.	Volume	Mass
Triton X-100	1 %
sodium deoxycholate	1 %
20 % SDS stock solution	0.1 %
NaCl Stock, 5 M	0.15 M
sodium phosphate, pH 7.2	0.01 M
Trasylol (1:100 dilution of what comes from Mobay)	1%
dH₂O

If Tris is substituted for phosphate, use 50 mmolar Tris-HCl, pH 7.2 .
Add sodium fluoride, EDTA and/or vanadate as necessary. Add the vanadate freshly each time you use it.