| Reagent | Final Conc. | Volume | Mass |
| dH2O | 400 ml | ||
| 20 x PBS Stock Solution | 1 x | 25 ml | |
| 0.5 M EDTA Stock Solution | 2% | 54 ml |
adjust volume to 500 ml, adjust pH to 7.2, sterile filter, store at 4°C
PBS / EDTA solution for non-enzymatic dissociation can also be purchased from GibcoBBRL (Versene, catalog #15040-066)