NZY broth - Top Agar - NZY plates - 2xYT media
| Reagent | Final Conc. | Volume | Mass |
| dH20 | 800 ml | ||
| NaCl (MW 58.44, Fisher S271-3) | 5 g | ||
| MgSO4 6H20 (MW 246.48, Fisher M63-500) | 2 g | ||
| Yeast Extract (DIFCO 0127-17-9) | 5 g | ||
| NZ Amine (casein hydrolysate, Sigma C-0626) | 10 g |
QS to 1 liter, autoclave
| Reagent | Final Conc. | Volume | Mass |
| dH20 | 800 ml | ||
| NaCl (MW 58.44, Fisher S271-3) | 5 g | ||
| MgSO4 6H20 (MW 246.48, Fisher M63-500) | 2 g | ||
| Yeast Extract (DIFCO 0127-17-9) | 5 g | ||
| NZ Amine (casein hydrolysate, Sigma C-0626) | 10 g | ||
| Agarose | 0.7% | 7 g |
QS to 1 liter, autoclave
| Reagent | Final Conc. | Volume | Mass |
| dH20 | 800 ml | ||
| NaCl (MW 58.44, Fisher S271-3) | 5 g | ||
| MgSO4 6H20 (MW 246.48, Fisher M63-500) | 2 g | ||
| Yeast Extract (DIFCO 0127-17-9) | 5 g | ||
| NZ Amine (casein hydrolysate, Sigma C-0626) | 10 g | ||
| Agar (DIFCO 0145-17-0) | 0.7% | 7 g |
QS to 1 liter, autoclave, cool on stirrer to ~50°C (o.k. when you can touch it), add antibiotics (e.g. 1ml 1000x tetracyclin stock per liter agar, pour (allow ~80 ml / 150 mm plate), let solidify, store upside down at 4°
| Reagent | Final Conc. | Volume | Mass |
| dH20 | 800 ml | ||
| NaCl (MW 58.44, Fisher S271-3) | 10 g | ||
| Yeast Extract (DIFCO 0127-17-9) | 10 g | ||
| Bacto-Tryptone | 16 g |
QS to 1 liter, autoclave