Acrylamide Gel, nondenaturing

Acrylamide Concentration % 3.5 5 8 12 20
Reagent Volume in mL
30% acrylamide 11.6 16.6 26.6 40 66.6
Water 67.7 62.7 52.7 39.3 12.7
5x TBE 20 20 20 20 20
10 % APS 0.7 0.7 0.7 0.7 0.7
TEMED 0.035 0.035 0.035 0.035 0.035

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