IPTG stock solution
Reagent
Final Conc.
Volume
Mass
IPTG
(Isopropyl Thio-beta-D-Galactoside, MW 238.31, Fisher BP1755-1)
1 M
0.2383 g
dH
2
O
0.8 ml
adjust Volume to 1.0 ml
sterile filter and store at -20°C
(dilute 1:10000 for indicator agar plates, e.g. 100 µl IPTG stock per 1 L agar solution,
dilute 1:1000 to soak nitrocellulose membranes for library screening)
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