| Reagent | Final Conc. | Volume | Mass |
| 1 M Tris-Cl pH 7.4 | 50 mM | 5 ml | |
| 5 M NaCl | 3 ml | ||
| 0.5 M EDTA pH 8.0 | 1 ml | ||
| Trasylol (10 K KIU / ml) | 1.0 ml | ||
| dH2O | 90 ml |
Reserve 50 ml of this buffer as a non-detergent immunoprecipitation wash buffer. To the other 50 ml add 62.5 µl Triton-X and 100 µl 10% SDS. This is the detergent immunoprecipitation wash buffer.