| Reagent | Final Conc. | Volume | Mass |
| dH2O | 400 ml | ||
| Triton X-100 | 1 % | 5 ml | |
| NaCl Stock, 5 M | 150 mM | 15 ml | |
| 1 M Tris-HCl pH 7.4 | 10 mM | 5 ml | |
| 0.5 M EDTA pH 8.0 | 1 mM | 1 ml | |
| EGTA (MW 380.4) | 1 mM | 0.19 g | |
| PMSF 0.1 M stock (toxic) | 0.2 mM | 1 ml | |
| NP-40 | 0.5 % | 2.5 ml |
If needed, add fresh sodium vanadate to final conc. of 0.2 mM (tyrosine phosphatase inhibitor).
| Reagent | Final Conc. | Volume | Mass |
| dH2O | 400 ml | ||
| Triton X-100 | 2 % | 10 ml | |
| NaCl Stock, 5 M | 300 mM | 30 ml | |
| 1 M Tris-HCl pH 7.4 | 20 mM | 10 ml | |
| 0.5 M EDTA pH 8.0 | 2 mM | 2 ml | |
| EGTA (MW 380.4) | 2 mM | 0.38 g | |
| PMSF 0.1 M stock (toxic) | 0.4 mM | 2 ml | |
| NP-40 | 1 % | 5 ml |
Add dH2O to 500 ml. If needed, add fresh sodium vanadate to final conc. of 0.4 mM (tyrosine phosphatase inhibitor).