HeBS buffer for Electroporation
(Hepes buffered saline)

Reagent Final Concentration Volume Mass
Hepes 20 mM   5.2 g
NaCl 137 mM   8 g
KCl 5 mM   0.373 g
dextrose 6 mM   1.09 g
Na2HPO4 0.7 mM   0.099 g
H2O   800 mL  

adjust pH to 7.05, QS to 1 L, sterile filter

Return to: Home - LabLinks - GH-PRL-PL