HBS (HEPES buffered saline)

Reagent	Final Conc.	Volume	Mass
dH₂O		900 ml
NaCl (MW 58.44)	150 mM		8.766 g
HEPES (MW 260.3)	20 mM		5.2 g

adjust volume with dH₂O to 1 liter, adjust pH to 7.0, autoclave if needed

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