HBHA (Hanks balanced salt solution with BSA, HEPES, Sodium Azide)

Reagent Final Conc. Volume Mass
dH2O   900 mL  
Hanks balanced salts (Sigma # H-2387)     1 Bottle
NaHCO3 (sodium bicarbonate, FW 84.01)     0.35 g
BSA (bovine serum albumin, fraction V) 0.5 mg / ml   0.5 g
NaN3 (sodium azide, MW 65.01) 0.1 %   1 g
HEPES ( FW 260.3) 20 mM   5.2 g

adjust pH to 7.0, adjust volume to 1 liter with dH2O, sterile filter, store at 4°C

Reagent Final Conc. Volume Mass
dH2O   1800 mL  
Hanks balanced salts (Sigma # H-2387)     2 Bottles
NaHCO3 (sodium bicarbonate, FW 84.01)     0.7 g
BSA (bovine serum albumin, fraction V) 0.5 mg / ml   1 g
NaN3 (sodium azide, MW 65.01) 0.1 %   2 g
HEPES ( FW 260.3) 20 mM   10.4 g

adjust pH to 7.0, adjust volume to 2 liters with dH2O, sterile filter, store at 4°C

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