Alkaline Phosphatase Buffer 1
(Glycine Buffer, 0.1 M, pH 10.4)

Reagent Final Conc. Volume Mass
dH2O   900 ml  
Glycine (free base, MW 75.07) 0.1 M   7.51 g
MgCl2, 1M Stock 1 mM 1 ml  
ZnCl2, 1M Stock 1 mM 1 ml  

adjust pH to to 10.4 with 10 N NaOH, QS to 1 L

Reagent Final Conc. Volume Mass
dH2O   900 ml  
Glycine (free base, MW 75.07) 0.1 M   7.51 g
MgCl2 (MW 203.31) 1 mM   0.203 g 
ZnCl2 (FW 136.3) 1 mM   0.136 g

adjust pH to to 10.4 with 10 N NaOH, QS to 1 L

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