Fischer's Medium
(for
Nb2 cells
)
Reagent
Final Conc.
Volume
Mass
dH
2
O
900 ml
Fischer's Medium Powder
(Sigma F-5008)
10.6 g (1 bottle)
Sodium Bicarbonate
1.125 g
2-mercaptoethanol
(14.2 M, Biorad 161-0710)
50 µM
3.5 µl
add dH
2
O to 1 liter,
adjust pH to 7.4,
sterile filter,
add 10 ml pen/strep (100x) to a final concentration of 100 U/ml penicillin, 100 µg/ml streptomycin,
for
MM
(maintenance medium) - add 10% FBS and 10% HS,
for
SM
(stationary medium) - add 10% HS
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