Denhardt's reagent, 50 x

Reagent Final Concentration Volume Mass
dH2O   400 ml  
ficoll (type 400, Pharmacia) 1 %   5 g
polyvinylpyrrolidone 1 %   5 g
bovine serum albumin (BSA, Fraction V, Sigma) 1 %   5 g

QS with dH2O to 500 ml. Mix overnight. Then filter through a disposable filter unit and freeze in 10 ml aliquots.

Return to: Home - LabLinks - GH-PRL-PL