Calcium Chloride for Competent Cells
Reagent
Final Conc.
Volume
Mass
1M CaCl2
60 mM
6 mL
80% glycerol
15%
18.75 mL
0.5 M PIPES pH 7
10 mM
2 mL
H
2
O
73.25 mL
Autoclave or sterile filter. Store at 4C.
Return to:
Home
-
LabLinks
-
GH-PRL-PL