| Reagent | Final Conc. | Volume | Mass |
| NBT (nitro blue tetrazolium, FW 817.6), toxic! | 0.33 mg/ml | 0.0033 g | |
| BCIP (5-bromo-4-chloro-3-indolyl phosphate, FW 370.4) | 0.17 mg/ml | 0.0017 g | |
| AP buffer 2 (Tris) | 10 ml |
| Reagent | Final Conc. | Volume | Mass |
| NBT (nitro blue tetrazolium, FW 817.6), toxic! | 0.33 mg/ml | 0.0165 g | |
| BCIP (5-bromo-4-chloro-3-indolyl phosphate, FW 370.4) | 0.17 mg/ml | 0.0085 g | |
| AP buffer 2 (Tris) | 50 ml |
| Reagent | Final Conc. | Volume | Mass |
| NBT (nitro blue tetrazolium, FW 817.6), toxic! | 0.33 mg/ml | 0.165g | |
| BCIP (5-bromo-4-chloro-3-indolyl phosphate, FW 370.4) | 0.17 mg/ml | 0.085 g | |
| AP buffer 2 (Tris) | 500 ml |
NBT dust is extremely toxic. Store at 4°C. Cover tubes with aluminum foil (both AP substrates are light sensitive).