Alkaline Phosphatase Buffer 2
(Tris-HCl, 100 mM, pH 9.5)

Reagent	Final Conc.	Volume	Mass
dH₂O		900 mL
Tris-HCl (MW 157.60)	100 mM		15.76 g
NaCl (MW 58.44)	100 mM		5.844 g
MgCl₂ (MW 203.31)	5 mM		1.015 g

adjust pH to 9.5, adjust volume with dH₂O to 1 liter, autoclave if needed

Return to: Home - LabLinks - GH-PRL-PL