Acrylamide Gel, denaturing

Acrylamide Concentration % 7.5 10 12 15
Reagent Volume in mL
30% Acrylamide 3.0 4.0 4.8 6.0
4x Tris-Cl-SDS, pH 8.8 3.0 3.0 3.0 3.0
H2O 6.0 5.0 4.2 3.0
10% APS 0.05 0.05 0.05 0.05
TEMED 0.01 0.01 0.01 0.01

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