Acetone / Formaldehyde Fixative

Reagent Final Conc. Volume Mass
dH2O   100 ml  
HEPES (MW 260.3) 20 mM   5.2 g
Formaldehyde solution 37% (FW 30.03) 3 % 40.5 ml  
Acetone (FW 58.08) 60 % 300 ml  

adjust pH to 7.0, adjust volume with dH2O to 500 ml, store at 4°C.

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