TDMN Buffer (for sequencing reaction)

Reagent Final conc. Volume Mass
TES free acid 0.28 M   0.064
conc. HCl 0.12 M 10 無  
1 M DTT 0.05 M 50 無  
1 M MgCl2 0.08 M 80 無  
5 M NaCl 0.2 M 40 無  
dH2O   820 無  

TES = N-tris[hydroxymethyl]methyl-2-aminoethanesulfonic acid, MW 229.2

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