| Reagent | Final Conc. | Volume | Mass |
| SDS | 10% | 50 g | |
| H2O | 400 mL |
Heat to 68C. Adjust pH to 7.2. Adjust volume to 500 mL with H2O. Sterile filter, if needed. Do not autoclave! (SDS will irreversibly precipitate)
| Reagent | Final Conc. | Volume | Mass |
| SDS | 20% | 100 g | |
| H2O | 400 mL |
Heat to 68C. Adjust pH to 7.2. Adjust volume to 500 mL with H2O. Sterile filter, if needed. Do not autoclave! (SDS will irreversibly precipitate)