Standard Protein Migration on SDS-PAGE Gels

Three ranges of BioRad standards are available: High Range (45,000 to 200,000), Low Range (14,400 to 97,400), Broad Range (6,500 to 200,000). In the following table each band is given in kD at its approximate location on a 7.5 %, 10 %, 12 %, or 15 % acryamide gel.

7.5% 10% 12% 15%
High Low Broad High Low Broad High Low Broad High Low Broad
200 200
200 200
116 116 116
97.4 97.4 97.4
116 116
200 200 97.4 97.4 97.4
66 66 66
200 200 66 66 66
116 116 116
97.4 97.4 97.4
45 45 45 45 45 45
116 116 66 66 66
31 31
97.4 97.4 97.4
31 31
21.5 21.5
45 45 45
66 66 66
14.5 14.5
21.5 21.5
31 31
45 45 45 6.5
14.5 14.5
21.5 21.5

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