Use BSA (bovine serum albumin) 1mg/ml stock solution (1ml
Eppendorf tubes) for standard curve.
Place 0, 2, 5, 10, 15, 20µg (=µl) BSA into plastic
cuvette. Use 2-5µl of sample.
Bring volume up to 800µl with H2O.
Add 200µl dye (Biorad Protein Assay Dye Reagent
Concentrate)
Cover cuvettes with parafilm and mix gently. The colour
of sample (intensity of blue) should be between the two
extremes of the standard curve. Avoid precipitation by
adding protein first, H2O second and dye last.
Measure protein concentration with spectrophotometer at
595 nm