YAC-1 (mouse lymphoma)

Species - Tissue - Features - Reference - Growth - Passage - Medium - Freeze - Source

Species: mouse

Tissue: lymphoma

Features: YAC-1 is a T cell lymphoma which was induced by inoculation of the Moloney leukemia virus (MLV) into a newborn A/Sn mouse. This cell line is sensitive to the cytotoxic activity of naturally occurring killer cells in mice (= NK cell target).

Reference:

• Kiessling, R., Klein, E., and Wigzell, H. "Natural" killer cells in the mouse. I. Cytotoxic cells with specificity for mouse Moloney leukemia cells. Specificity and distribution according to genotype. Eur.J.Immunol. 5(2):112-117, 1975. Medline

Mode of Growth: in suspension. For low background (spontaneous ⁵¹Cr release) in cytotoxicity assays, a viability of >95% is needed. This can be achieved by preventing overgrowth of the cells (passage every 2-3 days, especially on the day before the assay).

Method of Passage: dilution (usually 1 : 10)

Culture Medium: RPMI 1640 with 2 mM L-glutamine, 10% FBS

Freeze Medium: culture medium + 10% DMSO

Source: ATCC TIB-160

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