K562

Species - Tissue - Features - Reference - Growth - Passage - Medium - Freeze - Source

Species: human

Tissue: erythroleukemia (chronic myelogenous leukemia)

Features:

• preferred target for human NK-cells,
• tumorigenic in nude mice

Reference:

Lozzio, C.B. and Lozzio, B.B. Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome. Blood 45 (3):321-334, 1975. Medline

Mode of Growth: suspension

Method of Passage: Cultures can be maintained by the addition or replacement of fresh medium. Start new cultures at 1 X 10 exp5 viable cells/ml. Subculture at 1 X 10 exp6 cells/ml

Culture Medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mMsodium pyruvate, 90%; fetal bovine serum, 10%

Freeze Medium:

Source: ATCC (CCL-243)