Species: human
Tissue: choriocarcinoma
Mode of Growth: attached
Method of Passage: Trypsin-EDTA
Culture Medium: Eagle's minimum essential medium with non-essential amino acids and sodium pyruvate, 90%; FBS, 10%; antibiotic-free. Current medium for propagation: Eagle's MEM, 90%; FBS, 10%.
Freeze Medium: Culture medium, 95%; DMSO, 5%; antibiotic-free.
ATCC:
ATCC HTB-36, JEG-3 (Choriocarcinoma, human)
This is one of six clonally derived lines isolated and described
by Kohler and associates (J. Clin. Endocrinol. 32: 683-687, 1971;
Acta Endocrinol. Suppl. KBH 153: 137-153, 1971). Fragments of the
Woods' strain of the Erwin-Turner tumor in its 387th passage in
the hamster cheek pouch were used to set up explant cultures in
Ham's F10 medium with 13.5% horse and 3.2% FBS. Colonies were
isolated and recloned after propagation using irradiated feeder
layers of
human fibroblasts. JEG-3 released human chorionic gonadotropin,
human chorionic somatomammotrophin and progesterone; and was able
to transform steroid precursors to oestrone and oestradiol. A
culture at passage 103 was provided by the originator in 1975.
The line was propagated in Eagle's minimum essential medium with
15% FBS until transfer to the ATCC, when the supplement was
reduced to 10%.
CHARACTERISTICS REPORTED FOR TRANSFERRED STOCK
Grown as: Monolayer; transferred 1:2 weekly. Morphology:
Epithelial-like. In Vitro Cytopathology: (P106) Large malignant
tumor cells. Nude mouse: Forms malignant tumor consistent with
choriocarcinoma.
REFERENCE SEED STOCK PREPARED AT ATCC
Number of Serial Subcultures from Tissue of Origin: 124.
Freeze Medium: Culture medium, 95%; DMSO, 5%; antibiotic-free.
Viability: 95%. Karyology: Chromosome Frequency Distribution 50
Cells: 2n = 46 Cells: 2 2 4 5 17 10 10 Chromosomes: 67 68 69 70
71 72 73
This is a hypertriploid human cell line. The modal chromosome
number is 71, occurring at 34%, and polyploidy at 2.6%. The
t(4;11)(p15;q13), i(13q), t(10p15q), del(18)(q21), and 6 other
markers are common to most cells, and two other markers are found
in some. Giant satellites are seen in one N14, and two N22. N2,
N5, and N9 have 4 copies, and N7, N13, N18, N21 and X a single
copy. A single Y chromosome is detected by Q-band examination. Culture
Medium: Eagle's minimum essential medium with
non-essential amino acids and sodium pyruvate, 90%; FBS, 10%;
antibiotic-free. Isoenzymes: PGM3, 1-2; PGM1, 1; ES D, 1; AK1, 1;
GLO-1, 1-2; G6PD, B. Sterility: Tests for
mycoplasma, bacteria and fungi were negative.
Species: Confirmed as human by isoenzyme
analysis.