Species: African green monkey
Tissue: Kidney, SV40 transformed
Mode of Growth: attached
Method of Passage: Trypsin / EDTA
Culture Medium: DMEM + 10 % FBS
Freeze Medium: culture medium + 10 % DMSO
ATCC: ATCC CRL-1650 COS-1 (Kidney, SV40 transformed, African green monkey) Passage Frozen: Unknown. Current medium for propagation: Dulbecco's modified Eagle's medium, 90%; FBS, 10%. Additional Information: COS-1 is a fibroblast-like cell line established from CV-1 simian cells (ATCC CCL-70) which were transformed by an origin-defective mutant of SV40 which codes for wild-type T antigen. This line contains T antigen, retains complete permissiveness for lytic growth of SV40, supports the replication of ts A209 virus at 40C and supports the replication of pure populations of SV40 mutants with deletions in the early region. This line contains a single integrated copy of the complete early region of SV40 DNA. This is a suitable host for transfection, especially for vectors requiring expression of SV40 T antigen (ATCC 37192, 37193, 53100). Handle as potentially biohazardous material under at least Biosafety Level 2 containment. Reference: Cell 23: 175-182, 1981. Submitted by: Y. Gluzman, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY. Price Code: J