Species: human
Tissue: Transformed primary embryonal kidney
Features: 293 cells have rich glycosylation tools including GalNac-transferase. They are expected to be able to produce PLP-A in its specifically glycosylated way. An unsolved problem is the final sulfatation of GalNac (as in the pituitary), instead of attaching sialic acid to the end (as in the placenta).
Reference: J.Gen.Virol. 36: 59-72, 1977; Virology 77: 319-329, 1977; ibid., 86: 10-21, 1978
Mode of Growth: attached
Method of Passage: Trypsin - EDTA
Culture Medium: MEM + 10 % FBS (= Eagle's MEM with Earle's salts (e.g. Gibco BRL #11095 series) + 10 % fetal bovine serum); for culture in roller-bottles (roll-in incubator does not maintain 5% CO2) supplement with 20 mM HEPES
Freeze Medium: culture medium + 10 % DMSO
ATCC: ATCC CRL-1573 293 (Transformed primary embryonal kidney, human). Purified DNA from the cell line is also available as ATCC catalog number 45504 (25 ug) & 45505 (100 ug). The cost of the DNA from human cell lines is $75 and $150 for the 25 and 100 ug aliquots, respectively. Current medium for propagation: Eagle's MEM with Earle's BSS, 90%; heat-inactivated horse serum, 10%. Additional Information: The 293 cell line is a permanent line of primary human embryonal kidney transformed by sheared human adenovirus type 5 (Ad 5) DNA. The cells are particularly sensitive to human adenovirus, are highly permissive for adenovirus DNA, and contain and express the transforming genes of Ad 5. Handle as potentially biohazardous material under at least Biosafety Level 2 containment. The line has been used in the isolation of transformation defective, host-range mutants of Ad 5, and is excellent for titrating human adenoviruses. This is a hypotriploid human cell line. The modal chromosome number was 64, occurring in 30% of cells. The rate of cells with higher ploidies was 4.2 %. The der(1)t(1;15) (q42;q13), der(19)t(3;19) (q12;q13), der(12)t(8;12) (q22;p13), and four other marker chromosomes were common to most cells. Five other markers occurred in some cells only. The marker der(1) and M8 (or Xq+) were often paired. There were four copies of N17 and N22. Noticeably in addition to three copies of X chromosomes, there were paired Xq+, and a single Xp+ in most cells. References: J. Gen. Virol. 36: 59-72, 1977; Virology 77: 319-329, 1977; ibid., 86: 10-21, 1978. Submitted by: F.L. Graham, McMaster University, Hamilton, Ontario, Canada. Note: These cells are distributed for research purposes only. The McMaster University releases the line subject to the following: 1) 293 cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of the McMaster University. 2) Any proposed commercial use of these cells must first be negotiated with the Office of Research Contracts and Intellectual Property, 1280 Main Street West, Hamilton, Ontario, Canada L8S 4K1. Telephone: 905-525-9140 ext. 22873, FAX: 905-522-6066. Price Code: J Revised: December 1995