Station 6: Stain Slides

Pretreatment with trypsin produces dark and light areas on the chromosomes visible after being stained again with Giemsa. These bands allow for accurate classification and pairing of the chromosomes. This method is called G-banding.

To see chromosomes

• special stains applied - Band chromosomes by staining with Giemsa
• this stains the AT rich regions dark

Pretreatment

Giemsa staining