Fixed call suspensions are dropped onto slides in a temperature and humidity controlled chamber. As the cells dry, the membrane flattens and the chromosomes spread out. Consistent dry conditions are necessary for quality chromosome morphology. Slides are "aged" at 60 degrees C for at least 12 hours increase the quality and definition of the banding.
Although the procedures are methodical, the skill of the technologist is important to the quality of specimen preparation. Troubleshooting the variables within the process is an invaluable skill of an experienced technician.
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